Basic Information   [Help]    back to top..
Gene ID AT3G24650
Gene Name ABI3
Gene Type protein_coding
Description ABA INSENSITIVE 3 (ABI3); CONTAINS InterPro DOMAIN/s: Transcriptional factor B3 (InterPro:IPR003340); BEST Arabidopsis thaliana protein match is: AP2/B3-like transcriptional factor family protein (TAIR:AT3G26790.1); Has 4494 Blast hits to 2758 proteins in 300 species: Archae - 0; Bacteria - 132; Metazoa - 1104; Fungi - 405; Plants - 1155; Viruses - 101; Other Eukaryotes - 1597 (source: NCBI BLink).
GO Information   [Help] back to top
Gene IDAT3G24650
GO IDOntologyGO term
GO:0003677FunctionDNA binding
GO:0003700Functiontranscription factor activity
GO:0003700Functiontranscription factor activity
GO:0016563Functiontranscription activator activity
GO:0016563Functiontranscription activator activity
GO:0017163Functionbasal transcription repressor activity
GO:0006355Processregulation of transcription, DNA-dependent
GO:0009657Processplastid organization
GO:0009733Processresponse to auxin stimulus
GO:0009737Processresponse to abscisic acid stimulus
GO:0009790Processembryonic development
UniGene Information   [Help] back to top
Gene IDAT3G24650
UniGene IDAt.19959
Express Tissueseed
Expression Profile   [Help][Sample Description] back to top
Gene Structure   [Help] back to top
Gene Orientation: +           Gene Length: 3694
Gene Position on Chromosome: Start: 8997370     End: 9001063
UTR Position : AT3G24650.1: 1-541 3412-3694
Coding Region: AT3G24650.1: 542-2221 2422-2514 2660-2760 2894-2940 3058-3134 3247-3411
Protein Domain Information   [Help] back to top
Model ID: AT3G24650.1 Model Length: 721
PositionPfam DomainPfam IDScoreE-value
Mutant Information   [Help] back to top
Gene IDAT3G24650
Mutant Nameabi3-1
TAIR Stock NoTAIR Accession NoMutagen
CS241005161158ethylmethane sulfonate
Stock Description 
Phenotype"abscisic acid resistant, reduced sensitivity to ABA inhibition of germination, germinates in the presence of 10 microM ABA; increased level of endogenous ABA during seed development; does not exhibit compromised water relations, not wilty phenotype; reduced seed dormancy, fresh seeds germinate at high frequency; decreased sensitivity to the presence of GA biosynthesis inhibitors such as paclobutrazol; reduced sensitivity to salt and osmotic stress during germination; decreased accumulation of storage reserves and products of some late-embryogenesis abundant genes."
Gene IDAT3G24650
Mutant Nameabi3-4
TAIR Stock NoTAIR Accession NoMutagen
CS61301008493371ethylmethane sulfonate
Stock Description 
Phenotype"Abscisic acid resistant, strong allele, reduced sensitivity to ABA inhibition of germination, germination of seeds only slightly inhibited by 1mM ABA; normal plant growth; seeds fail to degrade chlorophyll during maturation keeping seeds green until mature."
Gene IDAT3G24650
Mutant Nameabi3-5
TAIR Stock NoTAIR Accession NoMutagen
Stock Descriptionseeds lose their viability within 5 weeks of storage at room temperature 
Phenotype"Abscisic acid resistant, strong allele, reduced sensitivity to ABA inhibition of germination, germination of seeds only slightly inhibited by 1mM ABA; normal plant growth; seeds fail to degrade chlorophyl during maturation keeping seeds green until maturity; absence of seed dormancy; reduced seed desiccation tolerance; reduced seed longevity; seeds lose their viability within 5 weeks of storage at room temperature."
Publication   [Help] back to top
Bies-Etheve N, da Silva Conceicao A, Giraudat J, Koornneef M, Leon-Kloosterziel K, Valon C, Delseny M. 1999. Importance of the B2 domain of the Arabidopsis ABI3 protein for Em and 2S albumin gene regulation. Plant Mol Biol. 40(6): 1045-54 [Abstract][PubMed ID: 10527428]

Rohde A, De Rycke R, Beeckman T, Engler G, Van Montagu M, Boerjan W. 2000. ABI3 affects plastid differentiation in dark-grown Arabidopsis seedlings. Plant Cell. 12(1): 35-52 [Abstract][PubMed ID: 10634906]

Nambara E, Hayama R, Tsuchiya Y, Nishimura M, Kawaide H, Kamiya Y, Naito S. 2000. The role of ABI3 and FUS3 loci in Arabidopsis thaliana on phase transition from late embryo development to germination. Dev Biol. 220(2): 412-23 [Abstract][PubMed ID: 10753527]

Riechmann JL, Ratcliffe OJ. 2000. A genomic perspective on plant transcription factors. Curr Opin Plant Biol. 3(5): 423-34 [Abstract][PubMed ID: 11019812]

Soderman EM, Brocard IM, Lynch TJ, Finkelstein RR. 2000. Regulation and function of the Arabidopsis ABA-insensitive4 gene in seed and abscisic acid response signaling networks. Plant Physiol. 124(4): 1752-65 [Abstract][PubMed ID: 11115891]

Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman BK, Yu G. 2000. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science. 290(5499): 2105-10 [Abstract][PubMed ID: 11118137]

Tamminen I, Makela P, Heino P, Palva ET. 2001. Ectopic expression of ABI3 gene enhances freezing tolerance in response to abscisic acid and low temperature in Arabidopsis thaliana. Plant J. 25(1): 1-8 [Abstract][PubMed ID: 11169177]

Nakamura S, Lynch TJ, Finkelstein RR. 2001. Physical interactions between ABA response loci of Arabidopsis. Plant J. 26(6): 627-35 [Abstract][PubMed ID: 11489176]

Suzuki M, Kao CY, Cocciolone S, McCarty DR. 2001. Maize VP1 complements Arabidopsis abi3 and confers a novel ABA/auxin interaction in roots. Plant J. 28(4): 409-18 [Abstract][PubMed ID: 11737778]

Brocard IM, Lynch TJ, Finkelstein RR. 2002. Regulation and role of the Arabidopsis abscisic acid-insensitive 5 gene in abscisic acid, sugar, and stress response. Plant Physiol. 129(4): 1533-43 [Abstract][PubMed ID: 12177466]

Lopez-Molina L, Mongrand S, McLachlin DT, Chait BT, Chua NH. 2002. ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination. Plant J. 32(3): 317-28 [Abstract][PubMed ID: 12410810]

Brocard-Gifford IM, Lynch TJ, Finkelstein RR. 2003. Regulatory networks in seeds integrating developmental, abscisic acid, sugar, and light signaling. Plant Physiol. 131(1): 78-92 [Abstract][PubMed ID: 12529517]

Brady SM, Sarkar SF, Bonetta D, McCourt P. 2003. The ABSCISIC ACID INSENSITIVE 3 (ABI3) gene is modulated by farnesylation and is involved in auxin signaling and lateral root development in Arabidopsis. Plant J. 34(1): 67-75 [Abstract][PubMed ID: 12662310]

Giraudat J, Hauge BM, Valon C, Smalle J, Parcy F, Goodman HM. 1992. Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell. 4(10): 1251-61 [Abstract][PubMed ID: 1359917]

Kroj T, Savino G, Valon C, Giraudat J, Parcy F. 2003. Regulation of storage protein gene expression in Arabidopsis. Development. 130(24): 6065-73 [Abstract][PubMed ID: 14597573]

Baumbusch LO, Hughes DW, Galau GA, Jakobsen KS. 2004. LEC1, FUS3, ABI3 and Em expression reveals no correlation with dormancy in Arabidopsis. J Exp Bot. 55(394): 77-87 [Abstract][PubMed ID: 14676287]

Monke G, Altschmied L, Tewes A, Reidt W, Mock HP, Baumlein H, Conrad U. 2004. Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA. Planta. 219(1): 158-66 [Abstract][PubMed ID: 14767767]

Kagaya Y, Toyoshima R, Okuda R, Usui H, Yamamoto A, Hattori T. 2005. LEAFY COTYLEDON1 controls seed storage protein genes through its regulation of FUSCA3 and ABSCISIC ACID INSENSITIVE3. Plant Cell Physiol. 46(3): 399-406 [Abstract][PubMed ID: 15695450]

Kagaya Y, Okuda R, Ban A, Toyoshima R, Tsutsumida K, Usui H, Yamamoto A, Hattori T. 2005. Indirect ABA-dependent regulation of seed storage protein genes by FUSCA3 transcription factor in Arabidopsis. Plant Cell Physiol. 46(2): 300-11 [Abstract][PubMed ID: 15695463]

Katagiri T, Ishiyama K, Kato T, Tabata S, Kobayashi M, Shinozaki K. 2005. An important role of phosphatidic acid in ABA signaling during germination in Arabidopsis thaliana. Plant J. 43(1): 107-17 [Abstract][PubMed ID: 15960620]

Nakashima K, Fujita Y, Katsura K, Maruyama K, Narusaka Y, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. 2006. Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis. Plant Mol Biol. 60(1): 51-68 [Abstract][PubMed ID: 16463099]

Bassel GW, Mullen RT, Bewley JD. 2006. ABI3 expression ceases following, but not during, germination of tomato and Arabidopsis seeds. J Exp Bot. 57(6): 1291-7 [Abstract][PubMed ID: 16531465]

To A, Valon C, Savino G, Guilleminot J, Devic M, Giraudat J, Parcy F. 2006. A network of local and redundant gene regulation governs Arabidopsis seed maturation. Plant Cell. 18(7): 1642-51 [Abstract][PubMed ID: 16731585]

Kotak S, Vierling E, Baumlein H, von Koskull-Doring P. 2007. A novel transcriptional cascade regulating expression of heat stress proteins during seed development of Arabidopsis. Plant Cell. 19(1): 182-95 [Abstract][PubMed ID: 17220197]

Tsukagoshi H, Morikami A, Nakamura K. 2007. Two B3 domain transcriptional repressors prevent sugar-inducible expression of seed maturation genes in Arabidopsis seedlings. Proc Natl Acad Sci U S A. 104(7): 2543-7 [Abstract][PubMed ID: 17267611]

Wang H, Guo J, Lambert KN, Lin Y. 2007. Developmental control of Arabidopsis seed oil biosynthesis. Planta. 226(3): 773-83 [Abstract][PubMed ID: 17522888]

Santos-Mendoza M, Dubreucq B, Baud S, Parcy F, Caboche M, Lepiniec L. 2008. Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis. Plant J. 54(4): 608-20 [Abstract][PubMed ID: 18476867]

Barrero JM, Talbot MJ, White RG, Jacobsen JV, Gubler F. 2009. Anatomical and transcriptomic studies of the coleorhiza reveal the importance of this tissue in regulating dormancy in barley. Plant Physiol. 150(2): 1006-21 [Abstract][PubMed ID: 19386806]

Pawlowski TA. 2009. Proteome analysis of Norway maple (Acer platanoides L.) seeds dormancy breaking and germination: influence of abscisic and gibberellic acids. BMC Plant Biol. 9: 48 [Abstract][PubMed ID: 19413897]

Nakashima K, Fujita Y, Kanamori N, Katagiri T, Umezawa T, Kidokoro S, Maruyama K, Yoshida T, Ishiyama K, Kobayashi M, Shinozaki K, Yamaguchi-Shinozaki K. 2009. Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy. Plant Cell Physiol. 50(7): 1345-63 [Abstract][PubMed ID: 19541597]

Jiang W, Yu D. 2009. Arabidopsis WRKY2 transcription factor mediates seed germination and postgermination arrest of development by abscisic acid. BMC Plant Biol. 9: 96 [Abstract][PubMed ID: 19622176]

Nambara E, Keith K, McCourt P, Naito S. 1994. Isolation of an internal deletion mutant of the Arabidopsis thaliana ABI3 gene. Plant Cell Physiol. 35(3): 509-13 [Abstract][PubMed ID: 8055176]

Parcy F, Giraudat J. 1997. Interactions between the ABI1 and the ectopically expressed ABI3 genes in controlling abscisic acid responses in Arabidopsis vegetative tissues. Plant J. 11(4): 693-702 [Abstract][PubMed ID: 9161030]

Parcy F, Valon C, Kohara A, Misera S, Giraudat J. 1997. The ABSCISIC ACID-INSENSITIVE3, FUSCA3, and LEAFY COTYLEDON1 loci act in concert to control multiple aspects of Arabidopsis seed development. Plant Cell. 9(8): 1265-77 [Abstract][PubMed ID: 9286105]

Sequence   [Help] back to top
CDS sequence   Protein sequence   cDNA sequence   Genomic sequence