Basic Information   [Help]    back to top..
Gene ID AT1G28300
Gene Name LEC2
Gene Alias LEAFY COTYLEDON 2
Gene Type protein_coding
Description LEAFY COTYLEDON 2 (LEC2); CONTAINS InterPro DOMAIN/s: Transcriptional factor B3 (InterPro:IPR003340); BEST Arabidopsis thaliana protein match is: AP2/B3-like transcriptional factor family protein (TAIR:AT3G26790.1); Has 515 Blast hits to 514 proteins in 42 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 515; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
 
GO Information   [Help] back to top
Gene IDAT1G28300
GO IDOntologyGO term
GO:0003677FunctionDNA binding
GO:0003700Functiontranscription factor activity
GO:0003700Functiontranscription factor activity
GO:0009790Processembryonic development
GO:0010262Processsomatic embryogenesis
GO:0010344Processseed oilbody biogenesis
GO:0010431Processseed maturation
GO:0010601Processpositive regulation of auxin biosynthetic process
GO:0045893Processpositive regulation of transcription, DNA-dependent
 
UniGene Information   [Help] back to top
Gene IDAT1G28300
UniGene IDAt.26290
Express Tissue
 
Expression Profile   [Help][Sample Description] back to top
 
Gene Structure   [Help] back to top
Gene Orientation: -           Gene Length: 3010
Gene Position on Chromosome: Start: 9896867     End: 9899876
UTR Position : AT1G28300.1: 1-38 2824-3010
Coding Region: AT1G28300.1: 39-533 691-765 1660-1760 1945-1991 2101-2174 2524-2823
 
Protein Domain Information   [Help] back to top
Model ID: AT1G28300.1 Model Length: 364
PositionPfam DomainPfam IDScoreE-value
170-274B3PF0236278.53.4e-23
 
Mutant Information   [Help] back to top
Gene IDAT1G28300
Mutant Namelec2-1
TAIR Stock NoTAIR Accession NoMutagen
CS38681005161417T-DNA insertion
Stock Description 
Phenotype"Embryo lethal, embryos not viviparous, cotyledons partially transformed into leaves, complex vascular pattern observed in cotyledons. Embryos are sensitive to abscisic acid. Mutant seeds were usually desiccation tolerant at maturity, although their viability was reduced slightly after several weeks of storage. Dry seeds often germinated to produce seedlings with distorted cotyledons. This defect resulted from degeneration of cotyledon tips prior to germination. Some trichomes form on cotyledons after germination and stomata are found on surface of cotyledons of mutant embryos The cotyledon surface on seedlings produced from dry seeds lacked trichomes (likely because any trichomes initiated during embryogenesis did not survive desiccation). Mutant seeds were consequently highly pigmented at maturity and anthocyanins were especially prominent in the top half of the mutant cotyledons. Mutant embryos do not form roots during the first 48 hours of culture. The pattern of storage product accumulation was altered, the hypocotyl was filled with mature protein bodies, while the cotyledons exhibited a gradient of starch and protein accumulation. Cells in the top half of mutant cotyledons were highly vacuolated, packed with starch grains, and lacked mature protein bodies, whereas cells closer to the hypocotyl contained less starch but many protein bodies. The accumulation of lipid bodies during embryogenesis was unusual too: distribution of lipid bodies was highly abundant in the hypocotyls of the mutant plants. Meinke, et al. (1994) | somatic embryogenesis ability is very limited in lec2 mutants Gaj MD, et al. (2005) | strongly impaired ability to induce somatic embryos in vitro "
 
Publication   [Help] back to top
Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman BK, Yu G. 2000. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science. 290(5499): 2105-10 [Abstract][PubMed ID: 11118137]

Stone SL, Kwong LW, Yee KM, Pelletier J, Lepiniec L, Fischer RL, Goldberg RB, Harada JJ. 2001. LEAFY COTYLEDON2 encodes a B3 domain transcription factor that induces embryo development. Proc Natl Acad Sci U S A. 98(20): 11806-11 [Abstract][PubMed ID: 11573014]

Meinke DW, Franzmann LH, Nickle TC, Yeung EC. 1994. Leafy Cotyledon Mutants of Arabidopsis. Plant Cell. 6(8): 1049-1064 [Abstract][PubMed ID: 12244265]

Curaba J, Moritz T, Blervaque R, Parcy F, Raz V, Herzog M, Vachon G. 2004. AtGA3ox2, a key gene responsible for bioactive gibberellin biosynthesis, is regulated during embryogenesis by LEAFY COTYLEDON2 and FUSCA3 in Arabidopsis. Plant Physiol. 136(3): 3660-9 [Abstract][PubMed ID: 15516508]

Gaj MD, Zhang S, Harada JJ, Lemaux PG. 2005. Leafy cotyledon genes are essential for induction of somatic embryogenesis of Arabidopsis. Planta. 222(6): 977-88 [Abstract][PubMed ID: 16034595]

Santos Mendoza M, Dubreucq B, Miquel M, Caboche M, Lepiniec L. 2005. LEAFY COTYLEDON 2 activation is sufficient to trigger the accumulation of oil and seed specific mRNAs in Arabidopsis leaves. FEBS Lett. 579(21): 4666-70 [Abstract][PubMed ID: 16107256]

Braybrook SA, Stone SL, Park S, Bui AQ, Le BH, Fischer RL, Goldberg RB, Harada JJ. 2006. Genes directly regulated by LEAFY COTYLEDON2 provide insight into the control of embryo maturation and somatic embryogenesis. Proc Natl Acad Sci U S A. 103(9): 3468-73 [Abstract][PubMed ID: 16492731]

Baud S, Mendoza MS, To A, Harscoet E, Lepiniec L, Dubreucq B. 2007. WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis. Plant J. 50(5): 825-38 [Abstract][PubMed ID: 17419836]

Stone SL, Braybrook SA, Paula SL, Kwong LW, Meuser J, Pelletier J, Hsieh TF, Fischer RL, Goldberg RB, Harada JJ. 2008. Arabidopsis LEAFY COTYLEDON2 induces maturation traits and auxin activity: Implications for somatic embryogenesis. Proc Natl Acad Sci U S A. 105(8): 3151-6 [Abstract][PubMed ID: 18287041]

Baud S, Dichow NR, Kelemen Z, d'Andrea S, To A, Berger N, Canonge M, Kronenberger J, Viterbo D, Dubreucq B, Lepiniec L, Chardot T, Miquel M. 2009. Regulation of HSD1 in seeds of Arabidopsis thaliana. Plant Cell Physiol. 50(8): 1463-78 [Abstract][PubMed ID: 19542545]

 
Sequence   [Help] back to top
AT1G28300.1
CDS sequence   Protein sequence   cDNA sequence   Genomic sequence