Basic Information   [Help]    back to top..
Gene ID AT1G21970
Gene Name LEC1
Gene Alias LEAFY COTYLEDON 1; EMB 212; EMB212; T26F17.20; T26F17_20
Gene Type protein_coding
Description "LEAFY COTYLEDON 1 (LEC1); FUNCTIONS IN: transcription activator activity, sequence-specific DNA binding transcription factor activity; INVOLVED IN: in 6 processes; LOCATED IN: nucleus, intracellular; EXPRESSED IN: seed, endosperm, cultured somatic embryo; EXPRESSED DURING: C globular stage, B proembryo stage, D bilateral stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Transcription factor, CBFA/NFYB, DNA topoisomerase (InterPro:IPR003957), Histone-fold (InterPro:IPR009072), Transcription factor CBF/NF-Y/archaeal histone (InterPro:IPR003958), Transcription factor, NFYB/HAP3, conserved site (InterPro:IPR003956); BEST Arabidopsis thaliana protein match is: nuclear factor Y, subunit B6 (TAIR:AT5G47670.2); Has 1463 Blast hits to 1463 proteins in 243 species: Archae - 2; Bacteria - 0; Metazoa - 492; Fungi - 348; Plants - 517; Viruses - 0; Other Eukaryotes - 104 (source: NCBI BLink)."
 
GO Information   [Help] back to top
Gene IDAT1G21970
GO IDOntologyGO term
GO:0009793Processembryonic development ending in seed dormancy
GO:0010262Processsomatic embryogenesis
GO:0045723Processpositive regulation of fatty acid biosynthetic process
 
UniGene Information   [Help] back to top
Gene IDAT1G21970
UniGene IDAt.28011
Express Tissuesilique
 
Expression Profile   [Help][Sample Description] back to top
 
Gene Structure   [Help] back to top
Gene Orientation: -           Gene Length: 2041
Gene Position on Chromosome: Start: 7727577     End: 7729617
UTR Position : AT1G21970.1: 1-46 1869-2041
Coding Region: AT1G21970.1: 47-98 1204-1868
 
Protein Domain Information   [Help] back to top
Model ID: AT1G21970.1 Model Length: 239
PositionPfam DomainPfam IDScoreE-value
62-127CBFD_NFYB_HMFPF00808126.68.2e-35
 
Mutant Information   [Help] back to top
Gene IDAT1G21970
Mutant Namelec1-1
TAIR Stock NoTAIR Accession NoMutagen
CS81011005161599T-DNA insertion
Stock Description 
Phenotype"Embryo lethal; viviparous embryos with cotyledons partially transformed into leaves; embryos sensitive to abscisic acid; embryos have reduced hypocotyl, cotyledons that remain green late in development; reduced protein and lipid bodies with starch being predominant storage product in mutant embryos. Trichomes form on cotyledons after germination; internal cells of cotyledons enlarge, vacuolate and resemble leaf mesophyll cells; intercellular spaces are prominant in cotyledons; enlarged shoot apical meristem; stomata are found on surface of cotyledons of mutant embryos; mature seeds are desiccation intolerant, however, immature seeds can be rescued before silique drying and germinated to produce seedlings. Root apex is active in viviparous seeds; immature mutant embryos germinated prior to desiccation produce plants that appear normal for other characteristics.| somatic embryogenesis ability is very limited in lec1 mutants."
Gene IDAT1G21970
Mutant Namelec1-2
TAIR Stock NoTAIR Accession NoMutagen
CS38671005161416T-DNA insertion
Stock Description 
Phenotype"Embryo lethal; viviparous embryos with cotyledons partially transformed into leaves; embryos sensitive to abscisic acid; embryos have reduced hypocotyl, cotyledons that remain green late in development; reduced protein and lipid bodies with starch being predominant storage product in mutant embryos. Trichomes form on cotyledons after germination; internal cells of cotyledons enlarge, vacuolate and resemble leaf mesophyll cells; intercellular spaces are prominant in cotyledons; enlarged shoot apical meristem; stomata are found on surface of cotyledons of mutant embryos; mature seeds are desiccation intolerant, however, immature seeds can be rescued before silique drying and germinated to produce seedlings. Root apex is active in viviparous seeds; immature mutant embryos germinated prior to desiccation produce plants that appear normal for other characteristics."
Gene IDAT1G21970
Mutant Namelec1-3
TAIR Stock NoTAIR Accession NoMutagen
CS57391005991849transposon insertion
Stock Description 
Phenotypeno phenotype information available at this time
 
Publication   [Help] back to top
Vicient CM, Bies-Etheve N, Delseny M. 2000. Changes in gene expression in the leafy cotyledon1 (lec1) and fusca3 (fus3) mutants of Arabidopsis thaliana L. J Exp Bot. 51(347): 995-1003 [Abstract][PubMed ID: 10948227]

Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman BK, Yu G. 2000. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science. 290(5499): 2105-10 [Abstract][PubMed ID: 11118137]

Brocard-Gifford IM, Lynch TJ, Finkelstein RR. 2003. Regulatory networks in seeds integrating developmental, abscisic acid, sugar, and light signaling. Plant Physiol. 131(1): 78-92 [Abstract][PubMed ID: 12529517]

Kroj T, Savino G, Valon C, Giraudat J, Parcy F. 2003. Regulation of storage protein gene expression in Arabidopsis. Development. 130(24): 6065-73 [Abstract][PubMed ID: 14597573]

Baumbusch LO, Hughes DW, Galau GA, Jakobsen KS. 2004. LEC1, FUS3, ABI3 and Em expression reveals no correlation with dormancy in Arabidopsis. J Exp Bot. 55(394): 77-87 [Abstract][PubMed ID: 14676287]

Henderson JT, Li HC, Rider SD, Mordhorst AP, Romero-Severson J, Cheng JC, Robey J, Sung ZR, de Vries SC, Ogas J. 2004. PICKLE acts throughout the plant to repress expression of embryonic traits and may play a role in gibberellin-dependent responses. Plant Physiol. 134(3): 995-1005 [Abstract][PubMed ID: 14963244]

Kagaya Y, Toyoshima R, Okuda R, Usui H, Yamamoto A, Hattori T. 2005. LEAFY COTYLEDON1 controls seed storage protein genes through its regulation of FUSCA3 and ABSCISIC ACID INSENSITIVE3. Plant Cell Physiol. 46(3): 399-406 [Abstract][PubMed ID: 15695450]

Gaj MD, Zhang S, Harada JJ, Lemaux PG. 2005. Leafy cotyledon genes are essential for induction of somatic embryogenesis of Arabidopsis. Planta. 222(6): 977-88 [Abstract][PubMed ID: 16034595]

To A, Valon C, Savino G, Guilleminot J, Devic M, Giraudat J, Parcy F. 2006. A network of local and redundant gene regulation governs Arabidopsis seed maturation. Plant Cell. 18(7): 1642-51 [Abstract][PubMed ID: 16731585]

Wenkel S, Turck F, Singer K, Gissot L, Le Gourrierec J, Samach A, Coupland G. 2006. CONSTANS and the CCAAT box binding complex share a functionally important domain and interact to regulate flowering of Arabidopsis. Plant Cell. 18(11): 2971-84 [Abstract][PubMed ID: 17138697]

Suzuki M, Wang HH, McCarty DR. 2007. Repression of the LEAFY COTYLEDON 1/B3 regulatory network in plant embryo development by VP1/ABSCISIC ACID INSENSITIVE 3-LIKE B3 genes. Plant Physiol. 143(2): 902-11 [Abstract][PubMed ID: 17158584]

Tsukagoshi H, Morikami A, Nakamura K. 2007. Two B3 domain transcriptional repressors prevent sugar-inducible expression of seed maturation genes in Arabidopsis seedlings. Proc Natl Acad Sci U S A. 104(7): 2543-7 [Abstract][PubMed ID: 17267611]

Wang H, Guo J, Lambert KN, Lin Y. 2007. Developmental control of Arabidopsis seed oil biosynthesis. Planta. 226(3): 773-83 [Abstract][PubMed ID: 17522888]

Santos-Mendoza M, Dubreucq B, Baud S, Parcy F, Caboche M, Lepiniec L. 2008. Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis. Plant J. 54(4): 608-20 [Abstract][PubMed ID: 18476867]

Mu J, Tan H, Zheng Q, Fu F, Liang Y, Zhang J, Yang X, Wang T, Chong K, Wang XJ, Zuo J. 2008. LEAFY COTYLEDON1 is a key regulator of fatty acid biosynthesis in Arabidopsis thaliana. Plant Physiol. : [Abstract][PubMed ID: 18689444]

Huang Y, Chen L, Wang L, Vijayan K, Phan S, Liu Z, Wan L, Ross A, Xiang D, Datla R, Pan Y, Zou J. 2009. Probing the endosperm gene expression landscape in Brassica napus. BMC Genomics. 10: 256 [Abstract][PubMed ID: 19490642]

Parcy F, Valon C, Kohara A, Misera S, Giraudat J. 1997. The ABSCISIC ACID-INSENSITIVE3, FUSCA3, and LEAFY COTYLEDON1 loci act in concert to control multiple aspects of Arabidopsis seed development. Plant Cell. 9(8): 1265-77 [Abstract][PubMed ID: 9286105]

Lotan T, Ohto M, Yee KM, West MA, Lo R, Kwong RW, Yamagishi K, Fischer RL, Goldberg RB, Harada JJ. 1998. Arabidopsis LEAFY COTYLEDON1 is sufficient to induce embryo development in vegetative cells. Cell. 93(7): 1195-205 [Abstract][PubMed ID: 9657152]

 
Sequence   [Help] back to top
AT1G21970.1
CDS sequence   Protein sequence   cDNA sequence   Genomic sequence